Testing antibotics on bacteria

Introduction Problem         To determine the effect of ten polar antibiotics on deuce different types of bacteria. I allow for effort six antibiotics on Escherichia coli, and six antibiotics on vitamin B complex subtilis. On Escherichia coli I will rill tetracycline, chloramphenicol, furadantin, nalidixic acid, triple sulfa, and kanamycin. On Bacillus subtilis I will test streptomycin, erythromycin, novobiocin, tetracycline, chloramphenicol, and penicillin. As a slope observation, I would too equal to see if Bacillus subtillis shows rampart to penicillin. The use of penicillin is being cut down because of the resistance many a(prenominal) types of bacteria are developing against it.          Hypothesis         My dead expect is that penicillin will inhibit the most growth against Bacillus subtilis, and that tetracycline will stop Escherichia coli more effectively than the others. Rationale         I f acial expression that this experiment is valid because it shows how different antibiotics oppose to different types of bacteria. It also points out the fact that not alone antibiotics work the kindred or that they work at alone on all types of bacteria. Materials 1 bottleful of tryptic soy agar-agar 1 culture of Escherichia coli 1 culture of Bacillus subtilis 4 stereotypic petri yellowish pinkes 1 pack of test discs 2 unfruitful pipettes 2 tubes of physiological saline 1 forceps 1 wax pencil 2 sterile swabs Method 1. tease apart the cap of the tryptic soy agar to allow it to vent. localise the bottle in a weewee bath at century degrees Centigrade so that the water level reached the level of the medium. The agar will melt in about 20 minutes. 2. piecemeal placid the medium to about 45 degrees Centigrade by allow the water bath cool. Then let the medium mould for 10 minutes. 3. Pour two plates for each species of bacteria. The agar should be most 5 mm deep. Cover each petri dish presently a! fter poring... If you want to get a teeming essay, target it on our website: BestEssayCheap.com

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